Optimization of Culture Conditions for Lipase Production by Pseudomonas aeruginosa ECS3

Lipases are hydrolytic enzymes with different biotechnological applications in food, detergents, pharmaceuticals, paper, and pulp industries. In lipase-producing bacteria, the encoding genes responsible for enzyme synthesis. Optimization of medium parameters is an essential factor large-scale production importance, this served as basis study. study, isolates ECS1, ECS3, ECS11, ECS14, ECS 19, ECS24, ECS28 were screened lipase on tributyrin agar plates. The highest isolate was identified by sequencing 16S rRNA region following a PCR procedure using specific primer. presence LipA gene confirmed polymerase chain reaction (PCR) amplification their primers. addition, some physical nutritional optimized production. On plates, ECS3 had zone hydrolysis (12mm) therefore selected. Based sequencing, producer Pseudomonas aeruginosa ECS3. This study also ascertained bacterium positive band 371 bp. Optimum activities observed at pH (8), temperature (35oC), incubation period (24 h). activity enhanced yeast extract (0.5% w/v), lactose agitation speed (120 rpm), 1% substrate concentration. showed influence culture conditions gene.